Precautions On Selection And Handling Of Mobile Phase - Hitachi CHROMASTER 5160 Instruction Manual

3.6.1 Precautions on Selection and Handling of Mobile Phase

Inflammation or injury due to toxic, corrosive or
stimulative solvent!
When using a toxic, corrosive or stimulative solvent, be
careful not to incur inflammation or injury. For details of
the properties of each solvent and how to handle it, refer to
Material Safety Data Sheet (MSDS). Be sure to handle
each solvent properly.
(a) Wear proper protective means (e.g., safety goggles)
so that a solvent will not come into direct contact with
the skin, eye, etc.
(b) Ventilate the laboratory room adequately to prevent
accidental inhalation of harmful solvent vapor.
(1) Pay enough attention to the characteristics of the mobile
phase to be used and a restriction on its operating
temperature.
(Refer to the organic solvent characteristics in Table 3-2.)
(2) For high sensitivity analysis with the UV detector, use a
solvent which is particularly low in UV absorption
characteristic.
chromatograph should be used. Generally used high-
grade methanol, ethanol, acetonitrile and other reagents
are significantly different in absorption characteristic at
shorter wavelengths, and their absorption characteristics
vary with production lot and manufacturer. Therefore, be
careful about using such reagents for high-sensitivity
analysis.
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WARNING
A commercially available solvent for liquid
3.6.1