Hitachi CHROMASTER 5160 Instruction Manual page 119

(4) Prepare other units to be connected to the autosampler as
described in the respective instruction manuals.
(5) Put the drain tube from the detector into the exclusive
detector waste solution bottle. If this tube is put into other
waste solution bottle such as for the Chromaster 5260
autosampler, baseline fluctuation may be caused due to
unstable back pressure.
(6) After confirmation of the above steps, connect the eluent to
the pump, and the detergent to the autosampler. Be sure
to use the mobile phase and detergent of the same
production lot.
(7) Start the pump to fill each flow path in the pump and
autosampler with fluid.
(8) After the mobile phase has reached the injection valve of
autosampler, turn on the autosampler power supply. If the
injection valve is switched over when no fluid is [INITIAL],
the valve seal may be damaged. To prevent this, be sure
to start the pump earlier.
The autosampler will go through the initialization sequence,
and then the [INITIAL] screen will appear.
(9) Press the WASH key of autosampler in order to replace the
detergent in the wash port with the fresh one and fill the
syringe with the detergent. Usually, the WASH key should
be pressed at least three times so that the old detergent in
the wash port can be completely replaced with the fresh
one. During this step, check to see if the detergent flows
normally, there are no leaks and the waste solution flows
into the waste bottle.
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