Hitachi CHROMASTER 5160 Instruction Manual page 806

Operating suggestions are indicated below.
Cut Injection Method
When applying the cut injection method, the loop
capacity more than the injection volume is required.
Select loop capacity within the area indicated in the
following figure.
The sample introduction volume is selected within the
shadow area of the following figure. (For example, 200
µL loop is required when measuring 100 µL sample.
This means that the sample volume requires the double
loop capacity.)
100
90
80
70
60
50
40
30
20
10
0
20 40 60
Fig. 2-7 Cut Injection Method
All Volume Injection Method
When applying all volume injection method, the loop
capacity much more than the injection volume is required.
Select loop capacity within the area indicated in the
following figure.
The sample introduction volume is selected within the
shadow area of the following figure. (For example, 200
µL loop is required when measuring 40 µL sample. This
means that the sample volume requires quintuple loop
capacity.)
2 - 10
80 100 120 140 160 180 200
Loop Capacity [µl]