Hitachi CHROMASTER 5160 Instruction Manual page 723

Where a flow rate at higher than 1.51 mL/min is used, turn
around the tube as shown in Appended Fig. 1-12 below.
When turning around, install the tube to the next groove with one
line of groove skipped.
Turn around with one
line of groove skipped
Appended Fig. 1-12 Assembling of Tubing (Where a Flow
APPENDIX 1 - 16
Rate at Higher Than 1.51 mL/min Is
Used)