Hitachi CHROMASTER 5160 Instruction Manual page 833

Syringe valve
4
vd
Plunger
wash
Syringe
Wash
fluid1
(2)
Buffer tube
Injection valve
3 2
1
5
P
3-way solenoid valve
1
D
vd :Dead volume (the instrument inside parameter)
vi :Injection volume (the sample net volume to the flow path)
vw :Waste volume (the air volume of the lead and rear part of a sample)
va :Air volume (the air volume which sample is sandwiched.)
Wash
fluid2
Aspiration of wash fluid1
The syringe aspirates [vd :Dead volume (inside parameter)] of
fluid1. Switch the syringe valve to the wash port side (port1)
after aspiration of the wash fluid1.
When [wash before sample aspiration] is set, refer to the
following operation:
Washing of the needle outer wall and the injection port flow path.
Deliver the wash fluid1 with the wash pump, move the needle
into the wash port, and wash the needle outer wall.
Wash the injection port flow path with the wash fluid1 after the
needle has moved into the wash port (the same as 2.3.3 Cut
injection method procedure (9)).
And when [fluid2 wash] is set, wash the injection port flow
paths using the wash fluid2 (the same as 2.3.3 Cut injection
method procedure (10)).
5
Loop
6
Waste
1
solution
From Pump To Column
2 - 37
Needle
Injection port
4
3
Waste
2
solution
Wash port
Rack
Sample