(10) Input the following contents as instrument conditions of
autosampler.
RACK CODE
SYRINGE SPEED
RACK PARAM
INJ.METHOD
LEAD VOLUME
REAR VOLUME
ASP SYRINGE SPEED : 2
DSP SYRINGE SPEED : 2
NEEDLE DOWN SPEED: 1
AIR VOLUME
NEEDLE WASH TIME SOLVENT1 : 15
RINSE PORT WASH TIME
SYRINGE VOL
(11) Set the pump flow rate to 0.1 mL/min.
(12) Set the detector wavelength to 265 nm.
Set the detector time constant to 0.5 s.
(13) Set the following conditions in the data processing unit.
SAMPLE PERIOD : 100 ms
STOP TIME
4 - 4
: 1
: 1
: RACK CODE = 1
X1 = 57.1
X2 = 178.6
Nx = 10
Y1 = 0.0
Y2 = 148.5
Ny = 12
Z = 48
P = 5
: CUT
: 10.0
: 50.0
: 2.0
: 1
: 2 (175 µL)
: 3 min