Hitachi CHROMASTER 5160 Instruction Manual page 577

Fig. 5-10 Observation of Ex Beam
(b) Fine adjustment
Following the coarse adjustment, set the excitation
(Ex) wavelength at 350 nm make a fine adjustment
while observing the data. Take the procedure given
below (refer to sections 3.1.1, 3.1.4).
(i) Re-mount the flow cell unit.
(ii) Press the
screen appears. Set an excitation (Ex)
wavelength.
<Wavelength setting screen (Ex indicated)>
Ex Em
350 350
Press numeric keys 3 5 0 and then the ENT key.
(iii) Emission (Em) wavelength will not be set here.
Leave the present setting as it is.
5 - 16
WL
key and the wavelength setting
(200 - 850.0)
Observe excitation
beam on paper strip