Hitachi CHROMASTER 5160 Instruction Manual page 770

Wash port
Appended Fig. 2-4 External View of Chromaster 5210
(3) Aspiration of Eluent
Eluent should be aspirated with the aspiration syringe
attached to the pump. The total internal volume (per flow
path) of the tubes for pump and the degassing chamber of
6-channel degassing unit is about 5 mL. So, eluent should
be aspirated in a few steps. When the proportioning valve
is used, eluent aspiration is required for each valve flow
path.
APPENDIX 2 - 4
Port 1
Autosampler
Port 2