Hitachi CHROMASTER 5160 Instruction Manual page 684

3.7 Preparation
3.7.4 Sample Pretreatment
(1) Filter samples without fail if they may contain fine dust.
Dust can clog column and deteriorate it.
(2) Remove foreign matter from samples without fail.
Foreign matter can lower the reliability of data, e.g.
generating abnormal peaks etc.
(a) Example of Filtering Samples
Dust in sample can be removed easily as follows.
Take sample into a syringe, attach cartridge type
0.45 μm membrane filter, and perform filtering.
See the figure at left.
NOTE: There are various ways of filtering sample.
Filter the sample in the way suitable for the
sample amount.
(b) Example of Removing Foreign Matter from
Sample
For example, when analysis is performed with a
reversed phase column (e.g. ODS column),
foreign matter can be removed as follows.
Take sample into a syringe, attach a normal
phase cartridge, and perform filtering.
figure at left.
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Filtering by cartridge
type 0.45 μm
membrane filter.
Filtered clean sample
See the