Hitachi CHROMASTER 5160 Instruction Manual page 113

Mobile phase container
Fig. 3-8 Degassing Mobile Phase by the Ultrasonic Cleaner
(a) Set mobile phase container well stirred into ultrasonic
cleaner.
* When using a mixed mobile phase, stir it
completely by using a stirrer, or else low
repeatability or baseline drift may be caused.
NOTE: Degas mobile phase of organic solvent by using
ultrasonic cleaner with water.
(b) Turn on the power of ultrasonic cleaner.
(c) When degassing, small bubbles are generated first,
then gradually larger bubbles are generated.
Continue degassing until bubbles are not generated
anymore. It takes 5 to 10 minutes to finish.
(d) When bubble generation finishes, turn off the power of
ultrasonic cleaner.
(e) Take out mobile phase container from ultrasonic
cleanser, wipe the water from the container, and set
the container to liquid chromatograph.
(2) Example of Degassing by Reducing Pressure
Example: Ethanol 20% + water 80%, 0.2% citric acid
aqueous solution (mobile phase is aqueous
solution)
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Remove cap
Ultrasonic cleaner