Sample Injection Procedure - Hitachi CHROMASTER 5160 Instruction Manual

2.3.3 Sample Injection Procedure

*3) Higher sample solubility solvent is used.
(Some types of samples are dissolved with 100% organic
solvent.)
NOTE: Stainless steel (SUS316), Vespel®, fluorine resin,
polypropylene (PP), ethylene-propylene rubber (EPDM)
and perfluoroelastomer materials are used for the wetted
parts of the instrument. Never use solvents that would
corrode these materials.
Cut Injection Method Procedure
The cut injection method, the injection volume, the lead
volume, the rear volume, and the air volume are restricted by
the following relational expression. A stroke of the syringe
capacity can't be used all.
<175 µL of Standard syringe >
vi + vl + vr + va ≦ 165 µL
vi :Injection volume
(The sample net volume to the flow path)
vl :Lead volume
(The disposal volume of the lead part of a sample)
vr :Rear volume
(The disposal volume of the rear part of a sample)
va :Air volume
(The air volume of the lead and rear part of a sample)
100 µL loop is used for the autosampler as the standard.
In general, the lead volume and the rear volume are set as 30
μl each when using 10 μl of sample.
2 - 13