Hitachi CHROMASTER 5160 Instruction Manual page 133

4.4.3 Poor Sensitivity, Poor Separation, Poor Reproducibility
Symptom
Poor sensitivity
Poor separation
Poor reproducibility of position
Poor reproducibility of area
Check position Possible cause
Connection piping
Leak from
Injection port
Leak from
Syringe valve
Autosampler
Leak from
Injection valve
Leak from Syringe
Dirty cleaning vessel,
sample container
Clogging of Needle
Dirty inside of Cell, Bubbles
Detector
in Cell, Leakage from Cell
Leakage from
Flow path
system piping connection
Deterioration of
Column
Column
Leakage from
Flow path
piping connection
system
Large variation of pressure
Environment Influence to Column
Unstable
Column oven
temperature
Leakage from
Autosampler
Autosampler
Flow path Leakage from the
system
Flow path system
Check
Check leak visually. Any Leakage?
Check leak visually.
Check leak visually.
Check leak visually.
Check leak visually.
Check Cell visually.
Check leak visually.
Check leak visually.
Check leak visually.
Result Remedy
Yes Tighten.
No OK
Tighten. Replace Injection port seal.
Yes
(See the instruction manual for Autosampelr.)
No OK
Replace the Valve seal.
Yes
(See the instruction manual for Autosampler.)
No OK
Yes Tighten.
No OK
Yes Replace the Syringe.
No OK
Clean or replace.
Clean the Needle.
See 4.4.1 "Cell".
Yes Tighten.
No
OK
See 4.4.1 "Cell".
Tighten.
Yes
No OK
See 4.4.2 "Abnormal pressure".
If Column oven is not used, large variation of
room temperature changes Peak.
Wait until the temperature becomes stable.
See "Autosampler" above.
Yes Tighten.
No OK
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