Hitachi CHROMASTER 5160 Instruction Manual page 270

1.1 Isocratic System
1) Mobile-phase liquid is delivered with the pump through the
degassing unit.
2) A sample is injected with the autosampler.
3) The injected sample is separated into its constituents with
the column maintained at a constant temperature by means
of the column oven.
4) The separated sample constituents are taken out as
analytical signals with the detector.
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