6 Performance tests
6.7 UV monitor U9-L test
6.7
UV monitor U9-L test
Method description
UV U9-L test checks the functionality of the UV monitor U9-L.
The method run takes approximately 10 minutes.
Required configuration
A correctly installed UV monitor U9-L is required to run the test.
Required material
The following materials are required:
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•
•
•
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Prepare the UV U9-L test
Follow the instructions to prepare the system before method start.
Step
1
2
3
4
5
260
Distilled water
1% acetone in distilled water
Ref 1 tubing, see Reference tubing, on page 439
Syringe, 25 to 30 ml
Mixer, 1.4 ml
Action
Direct outlet tubing W and W1 to a waste container.
Place inlet tubing A1 into distilled water.
Place inlet tubing B1 into a solution of 1% acetone in distilled water.
Connect the Ref 1 tubing on the Column valve position 1, to generate a back
pressure.
Make sure that the Mixer with a chamber volume of 1.4 ml is installed.
For information on how to change Mixer, see Section 7.8.2 Replace the Mixer,
on page 338.
ÄKTA avant User Manual 29035184 AE