GE AKTA avant User Manual page 221

Illustration of fraction marking using fixed volume fractionation with Fraction
collector F9-R
The illustration below shows the fractions collected, and the numerical marking of frac-
tions, when fixed volume (straight) fractionation is used with Fraction collector F9-R.
When fixed volume fractionation is used the delay volume is collected at the beginning
of fractionation.
Fraction collector F9-R saves the delay volume in the first tube (fraction 1), see the pre-
vious example illustration. Delay volumes are not indicated with a numerical fraction
mark on the chromatogram.
Note:
Note:
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The built-in fraction collector does not save the delay volume, instead the delay
volume is disposed of in the waste funnel.
The fractions collected by Fraction collector F9-R, are indicated by the prefix
2: (i.e. fractions, 2:2, 2:3 etc.).
5 Operation
5.8 Fractionation
5.8.3 Fractionation overview
Volume
Fractionation marks shown in chromatogram
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