Manual Instructions - Fraction Collection - GE AKTA avant User Manual

9.8.4

Manual instructions - Fraction collection

The following table describes the fraction collection related manual instructions available
for ÄKTA avant.
Instruction name
Fractionation
Peak fractionation
Stop fractionation
Stop peak fractionation
Last tube filled
Feed tube
ÄKTA avant User Manual 29035184 AE
9.8.4 Manual instructions - Fraction collection
Description
Is used when collecting fractions with a fraction collec-
tor.
Enables collection of only those peaks that fulfill the
conditions set in the Peak fractionation parameters
instruction.
Ends the fractionation after the set delay volume
(specified in the System Settings:Fraction collection:De-
lay volumes instruction) has been collected. The outlet
valve is then turned to position Waste.
Note:
If Stop fractionation is issued when both Fractionation
and Peak fractionation are active, fractionation is
stopped after the set delay volume has been collected.
The outlet valve remains in position Frac and peak frac-
tionation continues.
Ends the peak fractionation after the set delay volume
(specified in the System Settings:Fraction collection:De-
lay volumes instruction) has been collected. The outlet
valve is then turned to position Waste.
Last tube filled sets the action to perform after the
built-in fraction collector fills the last tube of the run:
pause the fractionation, direct the flow to one of the
outlet ports of the outlet valve or direct the flow to
waste.
Feed tube moves the fractionation arm of the built-in
fraction collector to the position specified by the param-
eter Start position, after the set delay volume has been
collected. A fraction mark is given in the chromatogram.
Fraction collector F9-R: Feed tube moves the tube rack
forward one tube after the set delay volume has been
collected and a fraction mark is set. When fractionation
or peak fractionation is not ongoing, Feed tube moves
the rack instantly and no fraction mark is set.
9 Reference information
9.8 Using Manual instructions
483