GE AKTA avant User Manual page 334

7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors
Step
9
10
Update the cell path length
Follow the instructions to define the UV 2nd cell path length. The flow cell path length
should be updated when the flow cell has been replaced or calibrated.
Step
1
2
3
4
334
Action
Enter the measured absorbance values into the table in the column UV-900
Absorbance (AU) in the UV-900 cell calibration Excel-file.
Note:
The values should be converted from mAU to AU.
When all absorbance values have been entered into the table, the real UV
flow cell path length is shown at the bottom of the table.
Note:
The regression coefficient R2 should be larger than 0.999. If this is not the
case, one or more measured values are faulty.
Action
In the System Control module, on the System menu, click Calibration.
In the Calibration dialog box, click UV 2nd cell path length in the Monitor
to calibrate list.
Enter the nominal flow cell path length in the Nominal length input field and
click Set.
If a calibration has been performed: enter the calculated flow cell path
•
length, obtained in the calibration procedure, in the Real length input
field and click Set.
If no calibration has been performed: enter the nominal flow cell path
•
length in the Real length input field and click Set.
Result: The UV flow cell path length is updated.
ÄKTA avant User Manual 29035184 AE