GE AKTA avant User Manual page 380

8 Troubleshooting
8.3 Troubleshooting: Monitors
Problem
Irregular pressure
curves
380
Possible cause and action
Air bubbles are passing through or are trapped in
•
the pump
Check that there is a sufficient volume of buffer
present in the flasks.
Check all connections and tubing for leaks and con-
strictions.
Check pump pressure curves. See Section 8.6 Trou-
bleshooting: Pumps, on page 397 for example of pump
pressure curves.
Purge the pumps, see Section 5.4.1 Prime buffer inlets
and purge system pumps, on page 166.
The check valve does not function correctly
•
Remove any solids in the valves by cleaning the check
valves according to the instructions in Section 7.6.9
Clean pump head check valves, on page 312.
Piston seal is leaking
•
Replace the piston seal according to the instructions
in ÄKTA avant Operating Instructions. A sign of leakage
is that the pump rinsing solution volume starts to in-
crease.
Blockage of flow path
•
Use the predefined method Prepare System to flush
through to clear blockage.
If necessary, replace tubing. See Section 7.8.1 Replace
tubing and connectors, on page 336.
Check the mixer inline filter. It can be clogged if unfil-
tered buffers or samples are applied. See Section 7.3.2
Replace the inline filter, on page 275 for instructions
how to replace the mixer inline filter.
Check the inlet filters. They can be clogged if unfiltered
buffers or samples are applied. To replace the filters,
see Section 7.8.7 Replace the inlet filters, on page 348.
Check the column. It can be clogged if unfiltered
buffers or samples are applied. To clean a column, see
Section 7.6.3 Perform Column CIP, on page 297.
ÄKTA avant User Manual 29035184 AE