GE AKTA avant User Manual page 192

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5.7 Sample application

5.7.1 Sample application using direct injection into the column

When preparing to

all the sample

in the Method editor, make the following selections

for the Sample Application phase of the method to

select Inject sample directly onto column, and

select Inject all sample using air sensor.

manually prime the sample inlet tubing with sample

or buffer, see Prime sample inlets, on page 173, and

make sure that the tubing is immersed in sample be-

fore starting the run.

make sure that the flow path from the sample inlet

valve up to the injection valve is filled with sample or

an appropriate buffer and that the buffer inlet tubing

is immersed in buffer.

ÄKTA avant User Manual 29035184 AE

Troubleshooting