GE AKTA avant User Manual page 486

9 Reference information
9.8 Using Manual instructions
9.8.4 Manual instructions - Fraction collection
Instruction name
Feed tube frac 2
Fractionation in outlet
valve
Stop frac in outlet valve
Peak frac in outlet valve
Stop peak frac in outlet
valve
Peak fractionation param-
eters
486
Description
During fractionation or peak fractionation the instruction
Feed tube frac 2 moves the second Fraction collector
tube rack forward one tube after the set delay volume
has been collected and a fraction mark is set. When
fractionation or peak fractionation is not ongoing, Feed
tube frac 2 moves the rack instantly and no fraction
mark is set.
Applicable if no fraction collector is used. Fractionation
in outlet valve enables fractionation via the outlet valve.
When the set fraction size/outlet has been collected,
the outlet valve turns to the next position. A fraction
mark is set in the chromatogram for each new outlet
position.
Applicable if no fraction collector is used. Stop frac in
outlet valve ends the fractionation in outlet valve after
the set delay volume (specified in System Settings:Tub-
ing and Delay volumes) has been collected. The outlet
valve is then turned to position Waste.
Applicable if no fraction collector is used. Peak frac in
outlet valve enables collection of only those peaks that
fulfill the conditions set in Peak fractionation parame-
ters. When the set fraction size/outlet has been collect-
ed, the outlet valve turns to the next position. A fraction
mark is set in the chromatogram for each new outlet
position.
Applicable if no fraction collector is used. Stop peak frac
in outlet valve ends the peak fractionation in outlet
valve after the set delay volume (specified in System
Settings:Tubing and Delay volumes) has been collected.
The outlet valve is then turned to position Waste.
Sets the detection parameters for peak collection, i.e. it
determines when a peak starts and ends. This informa-
tion is used by the instructions Peak fractionation, Peak
fractionation frac 2 and Peak frac in outlet valve in
order to start/end the peak collection.
ÄKTA avant User Manual 29035184 AE