GE AKTA avant User Manual page 469

Instruction name
Peak fractionation param-
eters
Delay volumes
The settings for system delay volumes are accessible as parameters to the Fraction
collection:Delay volumes instruction.
Parameter for
Delay volumes
Detector- Frac
Detector - Out-
let valve
Restrictor vol-
ume
pH cell volume
ÄKTA avant User Manual 29035184 AE
Description
The Peak fractionation parameters set the detection
parameters for peak collection, that is they decide when
a peak starts and ends. This information is used by the
instructions Fraction collection:Peak fractionation and
Fraction collection:Peak frac in outlet valve in order
to start/end the peak collection.
Description
Is used to define the delay volume between the monitor and the
built-in Fraction collector. The instruction is used to make sure
that the collected fractions correspond to the fractions indicated
in the chromatogram.
Is used to define the delay volume between the monitor and the
Outlet valve. The instruction is used to make sure that the collected
fractions correspond to the fractions indicated in the chro-
matogram. The delay volume must be changed when changing
tubing to another inner diameter or length, or when removing or
adding components.
Is used to calculate the delay volume between the monitor and
the Outlet valve. The instruction is used to make sure that the
collected fractions correspond to the fractions indicated in the
chromatogram. It is recommended not to alter the default values
for restrictor and pH cell delay volumes when standard modules
and standard tubing for flow restrictor are used.
Is used to calculate the delay volume between the monitor and
the Outlet valve. The instruction is used to make sure that the
collected fractions correspond to the fractions indicated in the
chromatogram. It is recommended not to alter the default values
for restrictor and pH cell delay volumes when standard modules
and standard tubing for flow restrictor are used.
9 Reference information
9.7 System settings
9.7.7 System settings - Fraction collection
469