GE AKTA avant User Manual page 179

Step
3
4
5
6
7
ÄKTA avant User Manual 29035184 AE
Action
Connect a 25 to 30 ml syringe to one of the purge valves of either of the
system pumps. Make sure that the syringe fits tightly into the purge connec-
tor.
Open the purge valve by turning it counterclockwise about 3 quarters of a
turn. Draw 10 ml of liquid into the syringe. Check that the Q1 inlet is filled
with liquid.
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
Repeat steps 2 to 5 for Q2, Q3 and Q4 respectively by setting the respective
Quaternary start concentration to 100%.
Tip:
The inlet tubing that is immersed in distilled water should be the last piece of
inlet tubing to be primed.
Tip:
If you will perform a BufferPro run, end with either Q1 or Q2.
Check that there is no air left in the pump by following the instructions in
Validate prime or purge of System Pump A or B or Sample Pump, on page 172.
If air bubbles are indicated, follow the instructions in Purge Quaternary Valve
and the system pumps, on page 180.
5.4 Prime inlets and purge pump heads
5.4.3 Prime Q inlets
5 Operation
179