Perform Column Cip - GE AKTA avant User Manual

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Perform Column CIP

Introduction

The Column CIP method is used to clean the column after purification runs, to remove

non-specifically bound proteins and to minimize the risk for carry-over between different

purification runs.

Maintenance interval

Perform a Column cleaning in place (Column CIP) when required, for example between

runs where different samples are used.

Required material

The following materials are required:

ÄKTA avant User Manual 29035184 AE

Hazardous substances. When using hazardous chemicals,

take all suitable protective measures, such as wearing protec-

tive clothing, glasses and gloves resistant to the substances

used. Follow local and/or national regulations for safe opera-

tion and maintenance of the product.

Hazardous chemicals during run. When using hazardous

chemicals, run System CIP and Column CIP to flush the entire

system tubing with distilled water, before service and mainte-

If hazardous chemicals are used for system or column cleaning, wash the

system or columns with a neutral solution in the last phase or step.

Appropriate cleaning solutions. Please refer to the instructions for use of the column.

7 Maintenance

7.6 Maintenance when required