GE AKTA avant User Manual page 374

8 Troubleshooting
8.3 Troubleshooting: Monitors
Problem
Waves on the gradient
Ghost peaks appear in
the gradient profile
Non-linear gradients or
slow response to %B
changes
Note:
Remember, conductivity
at high salt concentra-
tions are intrinsically not
linear.
374
Possible cause and action
Bad pump function
•
Check that the pump is operating properly. See Exam-
ples of pump pressure curves, on page 400 for example
of pump pressure curves.
Air in the flow path
•
Purge the pumps. See Section 5.4.1 Prime buffer inlets
and purge system pumps, on page166 and Section 5.4.3
Prime Q inlets, on page 178.
Poor mixing function
•
Check that the correct Mixer chamber size is used.
See Select Mixer chamber, on page 157 for recommen-
dations. To change the Mixer, see Section 7.8.2 Replace
the Mixer, on page 338.
Check the motor operation of the Mixer. Place a
magnet close to the mixer chamber during run. The
magnet should vibrate.
Check that the Mixer chamber is free from solids. To
open the mixer, see Section 7.3.2 Replace the inline
filter, on page 275.
A charged particle has been detected
•
Prepare the sample so that charged particles are
eliminated.
Air bubbles are passing through the flow cell
•
Check for loose tubing connections.
Use the Flow restrictor.
Dirt in the tubing
•
Make sure that the tubing has been washed properly.
Bad pump function
•
Make sure that the pump operates properly. See Ex-
amples of pump pressure curves, on page400 for exam-
ple of pump pressure curves.
The Mixer chamber is too large
•
Change to a Mixer chamber with a smaller volume.
See Select Mixer chamber, on page 157 for recommen-
dations. To change the Mixer, see Section 7.8.2 Replace
the Mixer, on page 338.
ÄKTA avant User Manual 29035184 AE