GE AKTA avant User Manual page 220

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5.8 Fractionation

5.8.3 Fractionation overview

Delay volume

The delay volume settings are used to make sure that the fractions collected during

fractionation, using the outlet valve or the fraction collector, correspond to the fractions

indicated in the chromatogram. The delay volume is the volume between the UV monitor,

and the fraction collector or outlet that is used, see the following illustration.

The time when a peak is detected

by the UV/Vis detector is called T

As the delay volume is affected by the length and diameter of the tubing, it should be

set according to the tubing and modules used, see Section 9.12 Delay volumes, on

Conductivity

Delay volume

Fraction collector

The peak reaches the fraction

collector at time T

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