GE AKTA avant User Manual page 531

E
Elution
predefined phase, 457
Equilibration
predefined phase, 458
Extension box, 53
F
Flow cells
conductivity, 98
pH, 80
UV, U9-L, 140
Flow path
component volumes, 509
delay volumes, 507
illustration, 432
prepare the flow path, 157
Flow restrictor
check function, 279, 281
replace flow restrictor, 347
Fractionation
assemble tube rack, 133,
210
delay volume, 220
fractionation types, 218
prepare, 210
Fraction collection settings, 468
Fraction collector
cleaning, 276, 301, 304
error codes F9-R, 419
F9-R test, 250
preparation, 210
quickrelease function, 91
requirements on deep well
plates, 94
requirements on tubes, 93
temperature control, 84
troubleshooting, 384, 394
Fraction collector F9-C
Cassettes and racks, 89, 203
Fraction collector F9-R
connect tubing, 134
Full scan, 85
G
Gel filtration
predefined method, 451
ÄKTA avant User Manual 29035184 AE
H
Hardware installation
of a module, 103
Holders
air sensor adapter, 49
bottle holder, 49
column clamp, 45
column holder, 44
column holder rod, 46
Extension box, 53
flexible column holder, 47
loop holder, 52
multi-purpose holder, 51
ordering information, 522
rail extension, 51
tubing holder comb, 48
tubing holder spool, 48
Hydrophobic interaction chro-
matography (HIC)
predefined method, 452
I
I/O-box E9
ÄKTA avant installation, 144
connectors, 145
description, 144
error codes, 420
I/O-box settings, 467
Important user information, 8
Injection valve
description, 70
flow paths, 71
Inlet tubing
prepare, 159
prime inlet tubing B, 166
Inlet valves
description, 64
Inlet valve A, 65
Inlet valve B, 65
Quaternary valve, 66
Sample inlet valve, 68
troubleshooting, 382
Installation
prepare waste tubing, 159
Install of optional modules, 102
Instrument control panel
error codes, 420
Instrument control unit
error codes, 415
Index
531