Function - GE AKTA avant User Manual

3 Standard instrument modules
3.10 Built-in fraction collector

3.10.1 Function

3.10.1 Function
Introduction
ÄKTA avant has a built-in fraction collector for safe and clean handling of fractions. A
cooling function protects the fractions from heat degradation.
Fractions can be collected in deep well plates and in tubes or bottles of different sizes.
Up to six cassettes for deep well plates and tubes can be used. The cassettes can be
used in any combination. Scanner functions identifies the types of cassettes and deep
well plates that are used.
Tubes and deep well plates are placed in cassettes which in turn are placed on the
cassette tray. The cassette tray is placed on the tray support. When the fraction collector
drawer is closed the cassette tray is inserted into the fraction collector chamber. A height
exclusion bar ensures that the tubes and deep well plates are correctly positioned and
cannot damage the dispenser head.
Temperature control
The built-in fraction collector has a temperature control function that provides an option
to set a desired fraction collector chamber temperature. The target temperature can be
set to any whole value between 6°C and 20°C. The default value is 20°C. Settings for the
temperature control function can be edited in the System Settings dialog box in System
Control, or in the Text Instructions pane in Method Editor.
Note:
Note:
Note:
Tip:
84
If the difference between the ambient temperature and the target temperature
is too big, it might not be possible to reach the target temperature.
If the temperature control function is turned off, the temperature in the fraction
collector chamber will increase to a temperature higher than the ambient
temperature.
If the cooling unit is turned on for longer periods of time at higher humidity,
there might be a buildup of ice inside the cooling unit. At higher humidity it is
recommended to turn off the cooling off between runs and have the drawer
open for ventilation.
The temperature in the fraction collector chamber can be viewed as a curve
in the chromatogram.
ÄKTA avant User Manual 29035184 AE