GE AKTA avant User Manual page 333

Step
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ÄKTA avant User Manual 29035184 AE
Action
Fit the syringe in the union Luer connector and inject the solution. DO NOT
remove the syringe.
Note:
Air trapped in the UV cell causes inaccurate measurements. To avoid introduc-
ing air into the UV cell, gently fill the union Luer up to the edge with test solu-
tion that is to be introduced, using the syringe. Then insert the syringe into
the union Luer.
Wait until the monitored absorbance value has stabilized.
In the Manual Instructions dialog box:
1 Select Monitors:Auto zero UV 2nd
2 Click Insert, then click Execute
Result: The UV absorbance is set to zero.
Remove the syringe.
Repeat the injections with the remaining four test solutions in increasing
concentration order. Use a new syringe for each solution.
After each injection, wait for a stable absorbance value. Note the measured
absorbance values for each solution.
7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors
333