GE AKTA avant User Manual page 161

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Plug unused valve ports

It is recommended to plug all unused valve ports with stop plugs before starting a run.

See Section 9.3 Tubing and connectors, on page 432 for information about connectors.

ÄKTA avant User Manual 29035184 AE

Cut the waste tubing from the Fraction collector and the Buffer tray to ap-

propriate length. It is important that the tubing is not bent and will not be

submerged in liquid during the run.

If the tubing is too short, replace it with new tubing. Do not lengthen the tubing

as this might cause obstruction of the tubing and flooding in the Frac chamber.

Make sure that the waste vessels will hold all the produced volume

of the run. For ÄKTA avant 25, a suitable waste vessel should typi-

cally have a volume of 2 to 10 liters. For ÄKTA avant 150, a waste

vessel should have a volume of 40 liters.

5.2 Prepare the flow path

Troubleshooting