System Test - GE AKTA avant User Manual

6 Performance tests

6.6 System test

6.6

System test

Method description
The System test checks the solvent delivery, the functionality of the UV and conductivity
monitoring systems, and the valve functionality of the standard system.
The method run takes approximately 30 minutes.
Required material
The following material is required:
•
•
•
•
•
•
Prepare the test
Follow the instructions to prepare the system before method start.
Step
1
2
3
4
5
256
Distilled water
1% acetone and 1 M NaCl in distilled water
Capillary loop Ref 1
Syringe, 25 to 30 ml
For ÄKTA avant 25: Mixer, 1.4 ml
For ÄKTA avant 150: Mixer, 5 ml
Action
Immerse the piece of inlet tubing marked A1 in distilled water.
Immerse the piece of inlet tubing marked B1 in 1% acetone and 1 M NaCl
in distilled water.
Immerse the piece of inlet tubing marked Buff in 1% acetone and 1 M NaCl
in distilled water.
Connect the capillary loop marked Ref 1 between Column valve ports 1A
and 1B to generate a back pressure.
For ÄKTA avant 25: Make sure that the Mixer with a chamber volume of
1.4 ml is installed.
For ÄKTA avant 150: Make sure that the Mixer with a chamber volume of
5 ml is installed.
For information on how to change Mixer, see Section 7.8.2 Replace the Mixer,
on page 338.
ÄKTA avant User Manual 29035184 AE