Sample Application Using A Superloop - GE AKTA avant User Manual

5 Operation
5.7 Sample application

5.7.2 Sample application using a Superloop

5.7.2 Sample application using a Superloop
Introduction
A Superloop allows injection of large sample volumes onto the column. A Superloop can
also be used for multiple injections, for example in a scouting experiment when the same
application conditions are required. Superloop models are available in 10 ml, 50 ml and
150 ml sizes.
A superloop can be connected to either the Injection valve or the Loop valve. When using
the Loop valve, up to five loops can be connected simultaneously.
Note:
Prepare the Superloop
To avoid injecting air into the system flow path, the Superloop should be prefilled with
buffer manually, before fitting the Superloop to the system.
Note:
Connect the Superloop
The following steps describe how to connect the Superloop to the Injection valve or to
the Loop valve.
Step
1
2
194
After loading a Superloop, always plug the Syr port on the Injection valve with
a Stop plug. With a Superloop connected to the valve, an over-pressure may
be created during injection.
Read the instruction for the Superloop to be used.
Action
Attach the Superloop to the instrument using a Column holder.
Connect a piece of tubing from the top of the Superloop to:
port LoopE on the Injection valve
•
or
an E port, e.g., 1E, on the Loop valve
•
ÄKTA avant User Manual 29035184 AE