GE AKTA avant User Manual page 97

Part
1
2
UV flow cells
UV flow cells are available with three different path lengths; 0.5 mm, 2 mm (default) and
10 mm.
Flow cells with shorter path lengths are suitable to use for high protein concentrations.
Flow cells with longer path lengths are suitable to use for low protein concentrations.
Note:
The real cell path length of the UV cell is automatically recognized by the monitor when
a cell is fitted. The UV data is normalized to the nominal path length. This allows UV data
from runs made with different UV flow cells (but with the same nominal path length) to
be directly compared.
The UV monitor U9-M is automatically calibrated every time the instrument is switched
on. The path lengths of the flow cells are factory calibrated and no further calibration is
needed.
ÄKTA avant User Manual 29035184 AE
Description
Inlet
UV flow cell
NOTICE
UV and conductivity flow cells on the high pressure side. When
placing UV and/or conductivity flow cells on the high pressure side
of the column, the UV flow cell has a maximum pressure limit of 2
MPa (20 bar) and the conductivity flow cell has a maximum pressure
limit of 5 MPa (50 bar).
If the UV flow cell with 10 mm path length is used, do not run at flow rates
higher than 50 ml/min as this flow cell increases the back pressure and may
result in a pressure higher than 2 MPa (20 bar).
3 Standard instrument modules
Part Description
3 UV detector
4 Outlet
3.11 UV monitor
97