Built-In Fraction Collector Test - GE AKTA avant User Manual

6.3

Built-in Fraction collector test

Method description
The Fraction collector test checks the functionality of the Fraction collector. For ÄKTA
avant 25, fractionation of 2 ml is performed in three sequential wells in each of two 96
deep well plates. For ÄKTA avant 150, fractionation of 20 ml is performed in three sequen-
tial tubes placed in each of two Cassettes for 50 ml tubes.
The method run takes approximately 10 minutes.
Note:
Required material
The following materials are required:
•
•
•
•
Prepare the Fraction collector
test
Follow the instruction to prepare the system before method start.
Step
1
2
3
ÄKTA avant User Manual 29035184 AE
The fraction collector test is evaluated manually and no report is generated.
Distilled water
Syringe, 25 to 30 ml
For ÄKTA avant 25:
-
Two Cassettes for deep well plates
-
Two 96 deep well plates
For ÄKTA avant 150:
-
Two Racks for 50 ml tubes
-
Twelve 50 ml tubes
Action
Immerse the piece of inlet tubing marked A1 in distilled water.
Prime inlet A1 and purge System pump A. See Section 5.4.1 Prime buffer inlets
and purge system pumps, on page 166.
Place the deep well plates or tubes in the Cassettes. Make sure that the deep
well plates are rotated so that the well marked A1 is positioned above the
A1 marking on the Cassette.
6 Performance tests

6.3 Built-in Fraction collector test

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