GE AKTA avant User Manual page 67

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Current mixing percentage for Q1-Q4 are shown in Process Picture in System Control.
The curves for Q1-Q4 can be shown in a chromatogram and stored in the result data
for documentation and for further studies in Evaluation.
Ports of Quaternary valve
The following illustration shows the ports of the Quaternary valve.
Port
Q1-Q4
A
B
BufferPro
The BufferPro tool allows automatic mixing of buffers during a run. Buffer preparation
can be performed using corresponding acid/base buffer systems. Buffer system, concen-
tration, pH and salt gradient are defined in the Method Editor.
Note:
When using BufferPro, immerse the Q inlets in the solutions according to the following
table.
Inlet
Q1
Q2
ÄKTA avant User Manual 29035184 AE
Description
Buffer inlets
Outlet to System pump A via Inlet valve A
Outlet to System pump B via Inlet valve B
In ÄKTA avant 25 BufferPro can be run at flow rates up to 25 ml/min. In ÄKTA
avant 150 BufferPro can be run at flow rates up to 40 ml/min.
Solution
Buffer stock solution
Acid or base
3 Standard instrument modules
Inlet
Solution
Q3
Water
Q4
Gradient salt stock solution
3.4 Inlet valves
67

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