GE AKTA avant User Manual page 328

7 Maintenance
7.7 Calibration procedures
7.7.3 Calibrate the Conductivity Monitor
Conductivity Monitor - user
calibration
Follow the instruction to calibrate the conductivity flow cell constant.
Step
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Action
Make sure that the instrument has been switched on for at least one hour.
In the System Control module, on the System menu, click Settings.
Result: The System Settings dialog box opens.
In the System Settings dialog, select Conductivity:Cond temp compensation.
Set the Compensation factor to 0, and click OK.
In the Calibration dialog box, in the Monitor to calibrate list, click Conduc-
tivity monitor - user calibration.
Prepare at least 25 ml of calibration solution. Use either a 0.1 M KCl certified
conductivity standard solution, or accurately prepare your own 1.00 M NaCl
solution.
Immerse a piece of sample inlet tubing, for example S1, in the calibration
solution.
Open the System Control module. On the Manual menu, click Execute
Manual Instructions.
Result: The Manual instructions dialog box opens.
In the Manual instructions dialog box:
Select Flow path:Injection valve, in the Position list, click Direct inject.
•
Click Insert.
Select Flow path:Column position, in the Position list, click By-pass.
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Click Insert.
Select Flow path:pH valve and set the Restrictor to In-line and the pH
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to Off-line. Click Insert.
Select Flow path:Sample inlet, select a position in the Position list (in
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this example S1). Click Insert.
Select Pumps and Pressures:Sample flow and enter 1.0 to ml/min in
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the Flow rate box. Click Insert.
Click Execute.
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Result: Calibration solution is pumped through the system by the Sample
pump.
Pump in at least 25 ml of the calibration solution, and wait until the conduc-
tivity signal is stable.
ÄKTA avant User Manual 29035184 AE