GE AKTA avant User Manual page 197

Step
7
8
Fill the Superloop using the
Sample pump
Follow the instruction below to fill the Superloop using the Sample pump.
Tip:
Step
1
2
3
ÄKTA avant User Manual 29035184 AE
Action
Load sample into the Superloop by emptying the syringe into the Injection
valve.
Disconnect the syringe and plug the Syr port with a Stop plug.
NOTICE
Glass tube splinter. Make sure to set the sample pressure below
the max pressure of the Superloop before executing a flow in the
Manual instructions dialog box when the Superloop is connected.
The Superloop can also be filled as part of a method run, as set in the Sample
Application phase in the Method Editor. For multiple injections, it may be
more convenient to fill the Superloop once, as described in the instruction be-
low.
Action
In the Manual instructions dialog box:
Select Flow path:Injection valve.
•
Select Sample pump load from the Position drop-down list.
•
Click Execute
•
Result: The Injection valve switches to Sample pump load position.
Make sure that the sample inlet tubing from the sample vessel is connected
to the Sample inlet valve.
In the Manual instructions dialog box:
Select Alarms:Alarm sample pressure.
•
Set Mode as Enabled.
•
Set a High alarm level that is below the maximum pressure of the Super-
•
loop.
Click Execute
•
5.7 Sample application
5.7.2 Sample application using a Superloop
5 Operation
197