GE AKTA avant User Manual page 258

6 Performance tests
6.6 System test
Faulty Step Response Result
Cause
If all values are faulty -
air in the pump or a
faulty pump
Faulty values at 5% -
damaged pump piston
seal in System pump B
Faulty values at 95% -
damaged pump piston
seal in System pump A
Faulty UV Absorbance Test
Cause
Incorrectly prepared
acetone solution
Faulty Pulsation Test
Cause
Air trapped in the
pumps
Faulty Conductivity Test Result
Cause
Incorrectly prepared
NaCl solution
258
Action
Air in pumps: Make sure to prime the buffer inlets and to
purge the System pumps and the Sample pump before
method start. See Section 5.4.1 Prime buffer inlets and
purge system pumps, on page 166 and Section 5.4.2 Prime
sample inlets and purge Sample Pump, on page 173.
Faulty pump. See Section 8.6 Troubleshooting: Pumps, on
page 397.
Replace piston seals. See ÄKTA avant Operating
Instructions, Maintenance chapter.
Replace piston seals. See ÄKTA avant Operating
Instructions, Maintenance chapter.
Action
Make sure that the acetone solution is 1% and that no
solution has evaporated.
Action
Make sure to prime and purge the system pumps before
starting the test, see Section 5.4.1 Prime buffer inlets and
purge system pumps, on page 166.
Action
Make sure that the NaCl solution is 1.00 M.
ÄKTA avant User Manual 29035184 AE