GE AKTA avant User Manual page 493

Instruction name
Column packing flow
Delay volume: Monitor to
outlet valve
Delay volume: Monitor to
frac
Delay volume: Monitor to
frac 2
Start volume count
ÄKTA avant User Manual 29035184 AE
9.8.7 Manual instructions - Advanced
Description
Is used to set flow rates over 25 ml/min and 150 ml/min
for ÄKTA avant 25 and ÄKTA avant 150, respectively.
Both A and B pumps are used to generate the flow,
making it possible to set flow rates up to 50 ml/min and
300 ml/min for ÄKTA avant 25 and ÄKTA avant 150, re-
spectively. Flow rate can be set either as volumetric or
as linear flow. A column type must be selected before
using linear flow.
Before executing the Column packing flow instruction
it is important to:
Immerse inlet tubing A1 and B1 in the same buffer
•
Disconnect the column outlet tubing from the Col-
•
umn valve and place the tubing in a waste vessel
Note:
When running Column packing flow only isocratic runs
can be performed, gradients cannot be generated.
Is used to define the delay volume between the monitor
and the Outlet valve. The instruction is used to make
sure that the collected fractions correspond to the
fractions indicated in the chromatogram. The delay
volume must be changed when changing tubing to an-
other i.d. or length or when removing or adding compo-
nents.
Is used to define the delay volume between the monitor
and the Fraction collector. The instruction is used to
make sure that the collected fractions correspond to
the fractions indicated in the chromatogram. The instruc-
tion is available only when the Fraction collector is se-
lected in the component list.
Is used to define the delay volume between the monitor
and the second Fraction collector. The instruction is
used to make sure that the collected fractions corre-
spond to the fractions indicated in the chromatogram.
The instruction is available only when the second Frac-
tion collector is selected in the component list.
Starts the volume counter function. The counted volume
is saved into a memory.
This instruction is best used in combination with Watch
instructions.
9 Reference information
9.8 Using Manual instructions
493