GE AKTA avant User Manual page 175

Step
9
Purge Sample Pump
If the priming was done thoroughly and the final buffer was drawn all the way into the
syringe and the validation of the priming showed that there was no air left in the pump
it is not necessary to purge the sample pump.
However, if the pressure signal indicated air bubbles left in the pump, follow the instruction
below to purge both the pump heads of the sample pump.
Step
1
2
3
4
ÄKTA avant User Manual 29035184 AE
Action
Check that there is no air left in the pump by following the instructions in
Validate prime or purge of System Pump A or B or Sample Pump, on page 172.
If air bubbles are indicated, follow the instructions in Purge Sample Pump,
on page 175.
Action
Make sure that all sample inlet tubing that is to be used during the method
run is immersed in the correct buffers.
Make sure that the waste tubing connected to injection valve port W2 is
immersed in a waste vessel.
Open the System Control module.
In the Process Picture:
Click the Injection valve icon, and then click Sample pump waste.
•
Result: The injection valve switches to waste position. This is necessary
to achieve a low back pressure during the purge procedure.
5.4 Prime inlets and purge pump heads
5.4.2 Prime sample inlets and purge Sample Pump
5 Operation
175