GE AKTA avant User Manual page 201

Tip:
Fill the sample loop using the
Sample pump
Most often the sample loop is filled using a syringe. However, to fill it using the sample
pump, follow the instructions for filling the Superloop, see Section 5.7.2 Sample application
using a Superloop, on page 194.
Note:
Empty the loop
General considerations
During the method run, the sample is automatically injected onto the column. The loop
is emptied and washed out using buffer from the system pumps. The total buffer volume
to be used for emptying and washing the loop is set in the Method Editor.
Using a sample loop connected to Loop valve V9-L or V9H-L
For maximum reproducibility, use complete loop fill when loading the loop, that is,
overfill the loop with a sample volume of up to 3-5 times the volume of the loop. For
minimum sample loss, use partial loop fill, that is, fill only up to 50% of the loop volume.
Empty the loop with 3-5 times the volume of the loop.
Volume used to empty a loop connected to Loop valve V9-L or V9H-L
To minimize the risk for carry over and to make sure that the complete sample volume
reaches the column, the loop should be emptied with an excess of buffer. The tubing
between the Loop valve port E and the Injection valve port LoopE holds a small volume.
If the loop is emptied with a volume equal to, or less than the loop volume this needs to
be taken into account. It is also important to use a low flow rate to ensure that the correct
volume is added to the column when injecting a small volume.
Note:
ÄKTA avant User Manual 29035184 AE
By default a union Luer female to 1/16 " male is used for connection of the
sample syringe to the injection valve. For loading of smaller volumes with
higher precision there is an alternative accessory, Fill port INV-907 (18-1127-
66) or the complete kit Injection Valve Kit (18-1110-89).
•
It is not necessary to set the Alarm Sample Pressure when filling a sample
loop.
•
It is recommended to overload the loop to make sure that the loop is
completely filled.
•
Set Flow rate to an appropriate value for the loop size.
•
After loading, plug the Syr port on the Injection valve with a Stop plug.
Partially emptying the loops that are attached to Loop valve V9-L or V9H-L
can increase the risk for carry over from one loop position to the next.
5.7 Sample application
5.7.3 Sample application using a sample loop
5 Operation
201