GE AKTA avant User Manual page 252

6 Performance tests
6.4 Fraction collector F9-R test
Possible causes of a failed test
The table below describes possible causes of a failed test. When possible sources of error
have been checked and corrected, repeat the test.
Cause
Incorrect volumes col-
lected in the tubes, and
disturbances of system
pressure curves:
•
•
Liquid collected in
wrong tubes:
Incorrect preparation of
buffer and tubing
252
Air trapped in Sys-
tem pump A
Faulty System pump
A
Action
Air in pumps: Make sure to prime inlet tubing A1 and purge
System pump A before method start, see Section 5.4.1
Prime buffer inlets and purge system pumps, on page 166.
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on
page 397.
Make sure the fraction collector delivery arm is positioned
above tube number 1 before starting the test.
Make sure that the system was correctly prepared, see
Prepare the Fraction collector F9-R test, on page 250.
ÄKTA avant User Manual 29035184 AE