GE AKTA avant User Manual page 200

5 Operation
5.7 Sample application
5.7.3 Sample application using a sample loop
Step
4
5
6
7
Tip:
200
Action
In the Process Picture:
Click on the Injection valve and select Manual load.
•
Result: Injection valve is set to manual load.
If the loop is connected to:
the Injection valve, continue to step 7.
•
the Loop valve, continue to step 6.
•
In the Process Picture:
Click the Loop valve.
•
Select the position the loop is connected to, for example Position 1.
•
Result: The Loop valve switches to the selected position.
Load sample into the sample loop. To avoid sample loss due to siphoning,
leave the syringe in the port until the sample has been injected onto the
column during the run.
It is recommended to overfill the loop to make sure that the loop is completely
filled. Excess of sample will leave the valve through port W1.
ÄKTA avant User Manual 29035184 AE