Sample Application Using Direct Injection Into The Column - GE AKTA avant User Manual

5.7.1

Sample application using direct injection into the column

Introduction
There are two ways to load sample directly onto a column:
•
•
Minimize sample loss
To minimize sample loss during direct injection of sample onto the column, sample re-
maining in the flow path will be pushed onto the column with buffer from the inlet valve.
This step is called Finalize sample injection in the text instructions of the sample appli-
cation phase of the method to be used. Refer to Section 5.9 Create a method and perform
a run, on page 225 for more information on methods and phases.
When preparing to
inject...
a fixed volume of sam-
ple
ÄKTA avant User Manual 29035184 AE
a fixed volume is loaded, or
all the sample is loaded.
5.7.1 Sample application using direct injection into the column
Then...
manually prime the sample inlet tubing with sample,
•
see Prime sample inlets, on page 173.
in the Method editor, make the following selections
•
for the Sample Application phase of the method to
be run:
select Inject sample directly onto column,
-
select Inject fixed sample volume and set the
-
volume to be injected.
make sure that the flow path from the sample inlet
•
valve up to the injection valve will be filled with an
appropriate buffer:
make sure that the buffer inlet tubing of the inlet
-
valve is immersed in buffer, and
enable the function Wash sample flow path with
-
buffer in the Sample application phase.
5 Operation
5.7 Sample application
191