GE AKTA avant User Manual page 450

9 Reference information
9.6 Predefined methods and phases
Predefined purification
method
Anion Exchange Chro-
matography (AIEX)
Cation Exchange Chro-
matography (CIEX)
450
Principle
After equilibration and sample application,
negatively charged proteins are adsorbed
to the column ligand. After a wash, to re-
move unbound sample, elution is per-
formed using a gradient of increasing salt
concentration ( e.g. NaCl). After column
wash and re-equilibration, a fraction collec-
tor accumulator wash is done to minimize
the risk for salt precipitation.
After equilibration and sample application,
positively charged proteins are adsorbed
to the column ligand. After a wash, to re-
move unbound sample, elution is per-
formed using a gradient of increasing salt
concentration (e.g. NaCl). After column re-
equilibration, a fraction collector accumu-
lator wash is done to minimize the risk for
salt precipitation.
Included phases
ÄKTA avant User Manual 29035184 AE