GE AKTA avant User Manual page 249

Possible causes of a failed test
The following table describes possible causes of a failed test. When possible sources of
error have been checked and taken care of, run the test once again.
Cause
Wrong volumes collect-
ed in the wells or tubes,
and disturbances on the
system pressure curves:
- Air trapped in System
pump A
- Faulty System pump A
Liquid is collected in the
wrong wells or tubes:
- Incorrect fractionation
settings
Liquid is collected in
wrong Cassette:
- Incorrect preparation
of the Fraction collector
Fractionation interrupt-
ed:
- Impurities on the Dis-
penser head of the
Fraction collector
Incorrect preparation of
buffer and tubing
ÄKTA avant User Manual 29035184 AE
Action
Air in pumps: Make sure to prime inlet tubing A1 and purge
System pump A before method start, see Section 5.4.1
Prime buffer inlets and purge system pumps, on page 166.
Faulty pump: See Section 8.6 Troubleshooting: Pumps, on
page 397.
Check the fractionation settings in the System Settings
dialog box.
Make sure that only two Cassettes are placed on the
Cassette tray, and that the Cassettes are positioned on
the cassette positions 1 and 6.
Clean the Fraction collector. See Section 7.6.4 Clean built-
in fraction collector, on page 301.
Make sure that the system was correctly prepared, see
Prepare the Fraction collector test, on page 245.
6 Performance tests
6.3 Built-in Fraction collector test
249