GE AKTA avant User Manual page 390

8 Troubleshooting
8.5 Troubleshooting: Fraction collector
Problem
The Frac chamber is
dark
The Frac waste is
flooded
The liquid leaving the
nozzle does not strike
the waste funnel
The Frac capillary is
blocked
The fraction volume
found in the tubes or
wells are smaller than
expected
390
Possible cause and action
The light has been turned off in UNICORN
•
Turn on the light in UNICORN. In System Control, select
System:Settings. Navigate to Fraction collector:Frac-
tion collector lamp and select Lamps On.
The lamp is broken
•
Contact Service.
The Frac waste tubing is positioned so that the flow
•
is obstructed
Untwist the waste tubing.
The waste container is placed in a position higher
•
than the waste outlet
Place the waste container in a position lower than the
waste outlet.
Waste tubing is blocked.
•
Clean or replace the waste tubing.
Check the position of the waste funnel
•
Refit the waste funnel.
Salt residuals in the capillary
•
Perform an Accumulator wash.
If persistent, contact Service.
Leakage on the wet side of the instrument
•
Localize the leakage and take care of the leakage, for
example by tightening connectors.
Air in pumps
•
Purge pumps. See Section 5.4.1 Prime buffer inlets and
purge system pumps, on page 166.
Bad pump function
•
See troubleshooting of pumps in Section 8.6 Trou-
bleshooting: Pumps, on page 397.
Leakage inside the Frac chamber
•
Contact Service.
ÄKTA avant User Manual 29035184 AE