GE AKTA avant User Manual page 373

Problem
Baseline drift of noisy
signal
Conductivity measure-
ment with the same
buffer appears to de-
crease/increase over
time.
ÄKTA avant User Manual 29035184 AE
Possible cause and action
Air in the Conductivity flow cell
•
Use the Flow restrictor.
Remove the air by flushing the flow cell with water or
buffer.
Leaking tubing connections
•
Tighten the connectors. If necessary, replace the
connectors.
Unclean Conductivity flow cell
•
Clean the Conductivity flow cell. See Section 7.6.12
Clean the Conductivity flow cell, on page 318.
Poor mixing function
•
Check the Mixer chamber size and change chamber
if necessary. See Section 7.8.2 Replace the Mixer, on
page 338.
Check the motor operation of the Mixer. Place a
magnet close to the Mixer chamber during run. The
magnet should vibrate.
Check that the Mixer chamber is free from solids. To
replace the inline filter, see Section 7.3.2 Replace the
inline filter, on page 275.
Unclean conductivity flow cell
•
Clean the Conductivity cell. See Section 7.6.12 Clean
the Conductivity flow cell, on page 318.
The ambient temperature may have decreased/in-
•
creased
Use a temperature compensation factor. The temper-
ature compensation factor is found in System Con-
trol:System Settings:Conductivity. Instruction regard-
ing the factor is also found in Section 7.7.3 Calibrate
the Conductivity Monitor, on page 327.
The Conductivity monitor needs to be calibrated
•
Check calibration with a solution with known conduc-
tivity.
Calibrate the Conductivity monitor. See Section 7.7.3
Calibrate the Conductivity Monitor, on page 327.
8 Troubleshooting
8.3 Troubleshooting: Monitors
373