GE AKTA avant User Manual page 451

Predefined purification
method
Chromatofocusing (CF)
Desalting
Gel filtration (GF)
ÄKTA avant User Manual 29035184 AE
Principle
After equilibration and sample application,
elution is performed using a pH gradient.
The proteins separate and elute according
to their isoelectric points. Finally, the col-
umn is re-equilibrated.
After equilibration and sample application,
the proteins are eluted isocratically. This
technique is commonly used for buffer ex-
change.
After equilibration and sample application,
proteins separate and elute according to
their size (largest first).
9 Reference information
9.6 Predefined methods and phases
Included phases
451