GE AKTA avant User Manual page 223

Fractionation settings for
Fraction collector F9-R
There are two fractionation settings for Fraction collector F9-R, DropSync off or DropSync
on .
Dropsync off: No synchronization of collection.
DropSync on: When using DropSync the sensors in the tube sensor detect when a drop
is released. The tube rack moves and positions the next tube under the tube sensor just
after a drop is released. Fractionation with DropSync can be used at flow rates up to 2
ml/min. For water and solutions with higher surface tension, a higher flow rate can be
used. Volatile solutions and solutions with low surface tension may require a lower flow.
Missing tubes or plates in built-in
fraction collector
When automatic cassette configuration is selected in the system settings the fraction
collector automatically detects which types of cassettes and plates that are present.
The fraction collector will however not detect if tubes or bottles are missing in the cas-
settes. Make sure that the cassettes to be used are occupied by appropriate types and
numbers of tubes or bottles before starting a run.
It is not possible to change the cassette configuration during a run. When the system
state is set to Pause it is possible to take out cassettes or plates from the fraction collector
only if they are replaced by cassettes or plates of the same type and are placed in the
same positions.
The action of the system when the last tube in the fraction collector is filled is set in the
instruction Last tube filled in the system settings. The flow can be directed to waste or
to any of the outlets or the run can be paused. If the action is set to Pause, the system
automatically pauses when the last tube is filled and prompts the user to replace the
filled tubes.
Missing tubes in Fraction
collector F9-R
If a tube is missing, Fraction collector F9-R will continue the fractionation on the tube
row located closer to the center of the fraction collector. The fractionation marks in the
chromatogram will then not reflect the tubes in which the sample is collected.
If the fraction collector runs out of tubes, the delivery arm moves to the fraction collector
center position while ÄKTA avant pauses and displays an error message.
ÄKTA avant User Manual 29035184 AE
5 Operation
5.8 Fractionation
5.8.3 Fractionation overview
223