GE AKTA avant User Manual page 458

9 Reference information
9.6 Predefined methods and phases
Phase Name
Equilibration
Manual Loop
Fill
Miscellaneous
Sample Appli-
cation
System CIP
System Prepa-
ration
Intelligent
Packing
458
Description
Equilibrates the column before purification, or re-equilibrates the
column after purification.
Is used to manually fill the additional sample application loops
mounted on the loop valve. The filling options are:
Partial loop fill
•
Complete loop fill
•
Can be added to any method at suitable places. The instructions
can help the user to better organize the graphical output of the
results or introduce a controlled delay in the method run.
Applies sample to the column. Defines the sample application
technique, the sample volume, and the handling of flowthrough.
Cleans the system after purification runs by rinsing the system
with a cleaning solution. One cleaning solution is used per phase.
Prepares the system before a run by removing storage solution
and filling the system and inlets with buffer solution. One prepara-
tion solution is used per phase.
A flow of hydraulic liquid pushes the adapter down. The user initi-
ates the start of compression at the exact point when the adapter
reaches the consolidated bed surface. The adapter compresses
the bed according to the packing factor or target bed height as
selected.
Only available for some systems and some types of AxiChrom
columns.
ÄKTA avant User Manual 29035184 AE